Images added / Abbildungen hinzugefügt 02.05.2019
Generally Sonography Atlas of Dogs →
Cardiovascular system → Pulmonary vessels
New subcategory added / Neue Unterkategorie hinzugefügt 02.05.2019
Generally Sonography Atlas of Dogs →
Cardiovascular system → Pulmonary vessels
Images added / Abbildungen hinzugefügt 01.05.2019
Generally Sonography Atlas of Dogs →
Cardiovascular system → Heart valvular diseases
Images added / Abbildungen hinzugefügt 01.05.2019
Generally Sonography Atlas of Dogs →
Cardiovascular system → Cerebral vessels
Images added / Abbildungen hinzugefügt 01.05.2019
Generally Sonography Atlas of Dogs → Lymphatic system → Hock
Schmidt AF; Nielen M; Klungel O; Hoes AW; de Boer A; Groenwold RHH; Kirpensteijn J; Amsellem P; Bacon N; Berg J; Kow K; Kurzman I; Maritato K; Moore A; Morello E; Sottnik J; Vail D "Prognostic
factors of early metastasis and mortality in dogs with appendicular osteosarcoma after receiving surgery: An individual patient data
meta-analysis ", Available online 3 September 2013. http://www.sciencedirect.com/science/article/pii/S0
Recently an aggregated data meta-analysis showed that serum alkaline phosphatase (SALP) and proximal humerus location are predictors for shorter survival in canine osteosarcoma. To identify additional prognostic factors of mortality and metastasis an individual patient data meta-analysis (IPDMA) was conducted. Individual patient data from
20 studies, identified via the VSSO society, were pooled. Univariable and multivariable hazard ratios (HR) for metastasis and mortality were assessed, using stratified Cox models. The study included 1405 dogs who received surgical treatment,of which thè metastasis status was measured in 1155 dogs and mortality status in 1336 dogs ; median survival was 256 days. High versus normal SALP and weight (kg) were associated with an increase in
hazard of metastasis [HR 1.34 (95%CI 1.07; 1.68) and HR 1.02 (per kg increase) (95%CI 1.01 ; 1.03)] and for mortality [HR 1.43 (95%CI 1.16; 1.77) and HR 1.02 (95%CI 1.01 ; 1.02)]. Distal radius tumor was associated with a lower hazard of metastasis compared to other locations: HR 0.75 (95%CI 0.58; 0.96). Proximal humerus and distal femur or proximal tibia location were related with an increased mortality: HR 1.53 (95%CI 1.26; 1.84) and HR 1.23 (95%CI 1.01; 1.49) compared to other locations. Older age (years) was associated with a higher hazard for mortality [HR 1.06 per year (95%CI 1.03 ; 1.09) ] but not for metastasis: HR 1.03 (95%CI 0.99 ; 1.06). These results confirm findings from a recent aggregated data meta-analysis and (in addition) showed that tumor location, SALP, weight were prognostic factors for both mortality and metastasis. Age was a prognostic factor for mortality but not for metastasis.
Osteosarcoma (OS) is a malignant tumor of mesenchymal origin that produces osteoid. Similarities between human and canine OS are striking and include thè bimodal age distribution, the high incidence of morbidity and mortality, the site of the tumor, histologic features and the response to the various treatment modalities (Withrow and Wilkins, 2010; Rowell et al., 2011). The biggest difference is that OS is much more common in dogs than in people. The
majority of canine primary bone tumors can be classified as OS, which predominantly occurs in large and giant breeds (Norrdin et al., 1989; Spodnick et al., 1992; Cooley and Waters, 1997; Ru et al., 1998; McNeill et al., 2007). OS dogs, treated only by amputation, have a median survival time of five months or less, with the majority succumbing to
metastatic disease (Brodey and Abt, 1976; Straw et al., 1991; Spodnick et al., 1992). Due to advances in disease management overall survival can be extended to 1 year (Straw et al., 1991). Given the increased treatment options, such as adjuvant chemotherapy, 'limb-sparing' surgery and radio-ablative methods, it has become even more important to differentiate between dogs with a worse and relatively improved prognosis. Numerous studies have explored the prognostic value of, for example gender, neuter status, age or serum alkaline phosphatase (SALP), but these studies have important limitations. Most notably, the relatively small number of patients included in these studies precludes precise estimation of the prognostic consequences of these factors. A possible solution for this is collecting and pooling reported prognostic associations from individual studies. Recently, Boerman et al. (2012)
conducted an aggregated data meta-analysis. This meta-analysis showed that elevated SALP and location of OS in the (proximal) humerus are associated with a shorter disease free survival time. However, as Boerman acknowledges, the included studies did not analyze SALP and tumor location consistently; some explored the univariable association, while other used multivariable methods. Furthermore, other characteristics, for example age, weight and neuter status, could not be analyzed because these were not reported by a sufficient number of studies.
An alternative to pooling the aggregated data is to acquire the individual patient data files. An individual patient data meta-analysis (IPDMA) permits for more uniform analyses with regard to follow-up time, categorization of variables, missing values and analysis methods used (Stewart and Parmar, 1993; Riley et al., 2010). Furthermore, individual patient data allows exploring associations not reported in the original publications. Consequently, such prognostic IPDMAs are powerful tools to identify prognostic factors and subgroups of patients with different prognoses. We conducted an IPDMA in order to estimate the independent prognostic value of gender, neuter status, age, weight,
breed, tumor location and SALP in predicting mortality or metastasis in canine OS.
2.1. Inclusion of individual patient data and assessment of data quality
To explore the relations between patient characteristic and (DF) survival we identified studies via the Veterinary Society of Surgical Oncology (VSSO). In January 2012 a call for collaboration was send out to VSSO members and other veterinary researchers. We attempted to include data from as many different researchers and institutes as possible. No a priori sample size calculations were performed. Data were deemed eligible if baseline patient chara
cteristics of OS dogs and time to event (death or metastasis) were recorded. To reduce the possibility of publication bias (Easterbrook et al., 1991), published and unpublished studies were both eligible. Impossible or unlikely data entries were explored and remaining irregularities were discussed with the original investigators. Data were collected on gender, neuter status, age (years), weight (kg), breed (Rottweiler, Golden Retriever, Labrador Retriev
er, Greyhound, Doberman, Irish Setter, mixed breeds, and other breeds), tumor location (proximal humerus, distal femur or proximal tibia, distal radius, and other locations), dichotomous SALP (using study specific cut-off values for high and normal SALP levels), surgery (limb-sparing, amputations), chemotherapy (no chemotherapy, cisplatin, lobaplatin or carboplatin, doxorubicin, doxorubicin combinations), and other treatments. To prevent low cell counts we refrained from using finer categories for breed, tumor location and chemotherapy. SALP status (at baseline) was dichotomized to follow clinical practice and because continuous SALP showed a positive linear sloped relationship
with the outcomes that stabilized to a flat slope at high SALP values. Patients who did not receive surgery, mostly due to euthanasia (n = 197), who received an infrequently used chemotherapeutic protocol (n = 13), or who received radiation therapy (n = 11) were excluded from all analyses.
2.2. Data analysis
To illustrate how patient characteristics were related to mortality or metastases at the clinically relevant time points of 5 and 12 months (Brodey and Abt, 1976; Straw et al., 1991; Spodnick et al., 1992), we stratified baseline characteristics according to the outcome status (mortality and metastasis) at these points. Univariable associations were estimated using a stratified Cox proportional hazards model (Harrell, 2001). All the models were stratified by study to account for possible differences in baseline hazard. If a variable was missing for a certain patient, that patient was excluded from the univariable analysis (i.e., listwise deletion).
We then performed a multivariable Cox proportional hazards analysis (stratified by study) to assess the independent associations between prognostic factors and outcome. Subjects were censored if they were lost to follow-up or died (censoring for mortality was only applied in models using the metastasis outcome). Associations are given as hazard ratios (HRs) with 95% confidence intervals (95%CI) and p-values using an alpha of 0.05. For categorical variables a p-value for trend was computed and the individual associations were only explored if this overall test was significant (i.e., p<0.05). All multivariable models were corrected for chemotherapy status. Variable selection for the multivari-able model was based on prior knowledge, no data driven selection method was used (i.e., stepwise selection). The proportional hazard (PH) assumption of the Cox models was checked based on Schoenfield residuals (Harrell, 2001). For the continuous variables weight and age linearity with the outcome was assessed using restricted cubic splines
plots (Harrell et al., 1996); relations appeared to be linear. To determine how well the multivariate models discriminate between subjects with a short time to event and subjects with a longer time to event, the c-statistic (i.e., area under the receiver operator characteristic curve) was calculated (Steyerberg et al., 2010). The c-statistic
represents the proportion of pairs of subjects where the subject with the longest observed time to event also received the longest predicted time to event; the c-statistic varies from 0.5 (no discrimination) and 1 (perfect discrimination).In the multivariable analysis missing values were imputed, across studies, based on the
areglmputation algorithm with ten imputations (Sterne et al., 2009; Harrell, 2012a). In each of the ten imputed datasets, a multivariable Cox proportional hazards analyses was conducted and results were pooled using Rubin's rule (Marshall et al., 2009). The study by Sottnik et al. (2010) (n = 69) did not provide information on time until death. Similarly, studies by Phillips et al. (2009) (n = 156) and Berg et al. (1997) (n = 94) did not record information on time to
metastasis. These studies were only used for the analyses they provided data for.
2.3. Sensitivity analysis
Effect estimates of the association between prognostic factors and non-mortality outcomes, such as metastasis, are potentially biased by competing risks. (Lau et al., 2009). In the case of time till metastasis a subject can be censored due to competing risks such as death (informative censoring). In such a case it is obviously wrong to assume that the subjects will get the event somewhere in the future (which is traditionally assumed when censoring). If this informative censoring is systematically related to a specific group (e.g., high SALP) censoring the deceased subjects inflates the cumulative incidence and competing risk occurs. Instead of censoring subjects who die before develo
ping a metastasis a competing risk analysis keeps these subjects in the denominator, decreasing the cumulative incidence. In canine OS, most subjects first experience a metastasis before dying, therefore the impact of competing risk by death is expected to be small. Nevertheless we conducted competing risk analyses to assess how much this impacted our results (Satagopan et al., 2004). We also assessed the impact of missing observations through a sensitivity analysis in which a multivariate analysis was conducted using only those subjects with completely
observed data. Additionally, to determine whether including subjects from small studies or unpublished studies biased our results we performed all analyses separately for large (50 or more subjects) and small studies (less than 50
subjects) and also stratified for publication status (i.e., if the study was published or not). To determine how influential the inclusion was of subjects who were not treated with chemotherapy, all analyses were also performed after excluding these patients. Finally, we assessed the impact of grouping lobaplatin and carboplatin in one group by repeated the analyses using separate categories for these chemotherapies.
All analyses were carried out with the R statistical package version 3.0.0 (R Development Core Team, 2013), the survival (Therneau, 2012), the rms (Harrell, 2012b) and the Hmisc (Harrell, 2012a) packages.
Data from 20 studies were included in this IPDMA, of which 11 studies were previously published (Kurzman et al., 1995; Berg et al., 1997; Kirpensteijn et al., 2002; Vail et al., 2002; Morello et al., 2003; Moore et al., 2007; Bacon et
al., 2008; Kow et al., 2008; Phillips et al., 2009; Sottnik et al., 2010). 19 studies reported solely on large breed dogs, while the unpublished study of Dr. Amsellem included 36 small breed canines. Characteristics of these studies are
presented in Table 1. Eighteen studies (1155 patients) provided data on metastasis status and nineteen studies (1336 patients) provided information on mortality.
3.1. Univariable analysis
At 5 months, in the 550 dogs without missing data, 153 dogs developed a metastasis (Table 2). High weight (per kg) was related to an increase in metastasis hazard: hazard ratio (HR) 1.02 (95%CI 1.00; 1.03). Compared to the category other tumor locations, the distal radius category was associated with a decrease in hazard: HR 0.40 (95%CI 0.23; 0.68). Elevated baseline SALP was associated with an increased hazard of metastasis: HR2.12 (95%CI 1.52; 2.95); see Figure Al for the Kaplan-Meier curves of SALP. Using other breeds as a reference Doberman subjects were related to a higher hazard, while mixed breed subjects were associated with a lower hazard: [HR2.16 (95%CI 1.06; 4.42) and HR 0.49 (95%CI 0.29; 0.84)]. By 1 year of follow-up the associations for metastasis of OS were similar to the results at 5 months (Table 2); median DF survival was 234 days.
The median survival was 256 days, based on the 598 dogs that had no missing data. At 5 months of follow up, the factors tumor location, breed and SALP at baseline were both univariable related to mortality and the magnitudes
of the observed relations were similar to those for metastasis. At 1 year, weight, location, breed and SALP showed similar and significant associations as found for metastasis at 1 year (table Al).
3.2. Multivariable analysis
After imputing missing values, 1155 subjects were available for the metastasis outcome (Table 3). By the end of follow-up 765 experienced a metastasis. Weight was associated with an increased hazard [HR 1.02 (per kg increase) (95% CI 1.01; 1.03)], as well as high SALP [HR 1.34 (95%CI 1.07; 1.68)]. Compared to other tumor locations, patients with a distal radius OS were associated with a decreased hazard of metastases: HR 0.75 (95%CI 0.58; 0.96). Furthermore, the proximal humerus location was associated with an increased hazard of metastases, however this association was not significant: HR 1.21 (95%CI 0.96; 1.53). Similarly, breed was no longer significantly associated with metastasis after adjusting for other baseline characteristics.
For the outcome mortality, 1336 dogs were available for analysis, of which 1076 died. The associations between weight and mortality, and SALP and mortality were similar to those found for the outcome metastasis (Table 3). Compared to the category other OS locations, proximal humerus tumors were associated with a higher hazard
of mortality: HR 1.53 (95%CI 1.26; 1.84). Similarly, having an OS at the distal femur or proximal tibia was related to an increased hazard: HR 1.23 (95%CI 1.01; 1.49). Finally, older aged subjects were also related to a higher hazard of mortality: HR 1.06 per year (95%CI 1.03; 1.09).
The discriminative performance of the multivariable models was modest: the model for the outcome metastasis had a c-statistic of 0.63, where as the model for the outcome mortality had a c-statistic of 0.61.
3.3. Sensitivity analysis
Fig. 1 shows the Kaplan-Meier survival curves for the outcome metastasis (with and without accounting for competing risks). Twenty nine (4%) subjects died without experiencing a metastasis event before dying. Consequently, ignoring competing risks had little impact on results: the standard Kaplan-Meier estimates only marginally overestimate the
cumulative incidence of metastases when accounting for competing risks.
Additional sensitivity analyses (on the impact of missing observations, size of the included studies, publication status of the included studies, and chemotherapy status of the dogs) did not show material difference compared to the results presented in Table 3. Results of these sensitivity analyses are available upon request.
In our IPD meta-analysis on prognostic factors for metastasis and mortality among dogs with OS, weight, SALP and tumor location were independently prognostic predictors of mortality as well as metastasis. Age was significantly related with mortality only.
In accordance with the "aggregated data" meta-analysis by Boerman et al. (2012), we found that elevated SALP was associated with a higher hazard of early mortality or metastasis. However, the Boerman study showed somewhat larger and less precise estimates [HR 1.62 (95%CI 1.21; 2.17) for mortality and 1.96 (95%CI 1.50; 2.56) for metastasis] compared to our results: HR 1.43 (95%CI 1.16; 1.77) and HR 1.34 (95%CI 1.07; 1.68). Compared to other tumor
sites, proximal humerus and distal femur or proximal tibia OS locations were related to an increased mortality hazard, but not metastasis. This is different from the Boerman study, which concluded that proximal humerus was significantly associated with both mortality and metastasis [HR 1.86 (95%CI 1.34; 2.57) and 2.53 (95%CI 1.34; 4.77)]. Furthermore, we found that having an OS tumor at the distal radius was as sociated with a decreased metastasis hazard.
Our IPDMA also showed that independent of breed, high weight increased the hazard of both metastases and mortality. Possibly, this is due to the crude categorisation of the breed variable, with a large "other" category resulting in unexplained variance. Also different from the findings of the Boerman study was that we found age to be significa
ntly related with mortality (increasing the hazard).
In this section we will discuss the limitation and strengths of our study. First, the number of patients with at least one missing observation was high (52% for the metastasis outcome and 57% for mortality). This was predominantly driven by SALP, which was only measured in 9 out of 20 studies. In aggregated meta-analyses, like the one conducted by Boerman et al. (2012), it is difficult to deal with missing data. In the current study we used an IPDMA design, which allows for imputation of missing values. Like all studies with missing observations, it is possible that missingness was
dependent not only on measured factors but also on unmeasured factors, thus results may still be biased even though missing data was multiple imputed. However, assuming that at least some of the missing values are dependent on measured factors, imputing missing values would likely decrease bias compared to a complete cases analysis. Second, several sensitivity analyses were performed, all showing similar associations as our main analysis,
confirming the robustness of our findings. Third, most studies used 1 or 2 specific chemotherapy regi mens, making it difficult to distinguish between chemotherapy effects and other study-specific influences. Thus, while it seems essential to include chemotherapy in modeling the independent prognostic associations between patient characteristics and outcomes, observed associations between chemotherapy and outcomes should not be interpreted causally. Fourth, none of the baseline variables, except chemotherapy (and only in some studies), were
randomly allocated. Therefore, it is possible that unmeasured or residual confounding influence our results. Given that it is impossible to randomly allocate baseline characteristics such as gender or a
ge, every study exploring these associations is potentially hampered by the possibility of confounding. Causal interpretation of observed associations might therefore lead to erroneous conclusions. For example, when, contrary to the association reported here, there is no causal relationship between weight and mortality (possible the reported association is due to some unmeasured protective genetic factor that is closely related to lower weight) intervening on weight will have no effect on the outcome. However, in such a situation (no causal relationship of weight) weight will still provide useful information on the baseline risk for the outcome. Thus, the importance of causality of the here
reported associations depends on the goal; either to intervene on risk factors or to use those factors for prognosti- cation. Fifth, meta-analyses can be subject to publication bias (i.e., bias due to including published studies only) (Easterbrook et al., 1991). By recruiting data via the VSSO network, about 40% of the included subjects were from unpublished sources, making the potential for publication bias smaller. On the other hand, some researchers did not respond to our requests for collaboration therefore results presented here do not include all possible data and we cannot rule out the possibility that inclusion of more data could change our estimations. Finally, the discriminative ability of all models was modest. Including clinical predictors like grade or type of tumor could potentially increase
this discriminative ability. However, in the current study this was impossible due to the large numberof studies that did not record data on these variables.
Our present study used relatively new study techniques to combine individual patient data from different sources termed individual patient data meta-analysis (IPDMA). While an IPDMA requires big investments regarding time and resources we believe that the opportunities of using individual patient data compared to the alternative of relying
on aggregated data outweigh this burden. An advantage of conducting an IPDMA is that one can explore relations not reported by the original authors. In our case this allowed us to estimate 7 associations while the Boerman aggre- gated data meta-analysis (Boerman et al., 2012) could only explore 3 associations. Similarly, IPMDA techniques ensure that when one wants to conduct multivariable analysis all estimates are corrected for the same set of variables. Without the same corrections, one has to rely on the reported estimates and as Boerman showed it is likely that every study uses distinct sets of covariables. A third advantage is that one can uniformly recode the data this can be particularly important if different cut off values or reference categories are used by the original authors (e.g., categorizing age using a cut point of 5 or 7 years). Fourth, IPDMAs also allows one to check model assumption such as linearity or proportional hazard. Lastly by having access to individual patient data one can more easily perform subgroup analysis, sensitivity analysis and apply more refined methods to deal with problems such as missing values or competing risks. However, while we strongly recommend researcher to use IPD techniques in meta-analyses one should remember that the success of any meta-analysis ultimately depends on quality of the original data.
In conclusion, the IPDMA design used in this study allowed us to assess prognostic factors in canines with osteosarcoma. We identified weight, SALP, and tumor location as independent prognostic factors of metastasis and mortality, while age was only associated with early mortality. This study design allowed for the application of advanced missing data techniques and multiple sensitivity analyses and showed the necessity to use individual participant data in order to comprehensively assess prognostic factors in this field of research.
Conflict of interest
None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.
AFS, MN, RHHG, JK contributed to the idea and design of the study. AFS and JK approached and coordinated with researchers to collect data. AFS performed the analyses and drafted the manuscript. MN, OHK, AWH, AB, RHHG, the VSSO investigators and JK provided guidance during initial planning of the paper and during critical revision. AFS had full access to all of the data and takes responsibility for the integrity of the data presented.
This work was supported by Research Focus Areas funding of the Utrecht University, which is a collaboration between the faculties of medicine, science, and veterinary medicine. The study sponsor was not involved in the design, collection, analysis or writing of this manuscript.
Prior postings and presentations
This study and its results have not been previously published, neither has it been presented at conferences.
We gratefully wish to acknowledge the following researchers for their willingness to collaborate:
Dr. Pierre Amsellem; Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
Dr. Nicholas Bacon; College of Veterinary Medicine, University of Florida, Gainesville,Florida, US.
Professor Dr. John Berg; Tufts Cummings School of Veterinary Medicine, North Grafton, MA, US.
Dr Kelvin Kow; College of Veterinary Medicine, University of Florida, Gainesville, Florida, US.
Dr. Ilene Kurzman; School of Veterinary Medicine University of Wisconsin, Madison, Wisconsin, US.
Dr. Karl Maritato, MedVet Medical and Cancer Center for Pets, Cincinnati, Ohio, US.
Dr. Antony Moore; Veterinary Oncology Consultants, Australia.
Dr. Emanuela Morello; School of Veterinary Medicine, Turin, Italy.
Dr Joe Sottnik; Animal Cancer Center, Colorado State University, Fort Collins, CO, US.
Professor Dr. David Vail; School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin, US.
Finally we wish to thank the Veterinary Society of Surgical Oncology (VSSO) society for allowing us to approach their members for collaboration.
Appendix A. Supplementary data
Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/ j.prevetmed.2013.08.011.
Bacon, N.J., Ehrhart, N.P., Dernell, W.S., Lafferty, M., Withrow, S.J., 2008. Use of alternating administration of carboplatin and doxorubicin in dogs with microscopic metastases after amputation for appendicular osteosarcoma: 50 cases (1999-2006). J. Am. Vet. Med. Assoc. 232, 1504-1510.
Berg, J., Gebhardt, M.C., Rand, W.M., 1997. Effect of timing of postoperative chemotherapy on survival of dogs with osteosarcoma. Cancer 79, 1343-1350.
Boerman, I., Selvarajah, G.T., Nielen, M., Kirpensteijn, J., 2012. Prognostic factors in canine appendicular osteosarcoma - a meta-analysis. BMC Vet. Res. 8, 56.
Brodey, R.S., Abt, DA, 1976. Results of surgical treatment in 65 dogs with osteosarcoma. J. Am. Vet. Med. Assoc. 168,1032-1035.
Cooley, D.M., Waters, D.J., 1997. Skeletal neoplasms of small dogs: a retrospective study and literature review. J. Am. Anim. Hosp. Assoc. 33, 11-23.
Easterbrook, P.J., Berlin.JA, Gopalan, R., Matthews, D.R., 1991. Publication bias in clinical research. Lancet 337, 867-872.
Harrell Jr., F.E., 2001. Regression Modeling Strategies: With Applications to Linear Models, Logistic Regression and Survival Analysis. Springer, New York.
Harrell, F.E., Jr., 2012. Hmisc: Harrell Miscellane ous. R package.
Harrell F.E., Jr., 2012. rms: Regression Modeling Strategies, R package.
Harrell Jr., F.E., Lee, K.L., Mark, D.B., 1996. Multivariate prognostic models: issues in developing models, evaluating assumptions and adequacy, and measuring and reducing errors. Stat. Med. 15,361-387.
Kirpensteijn, J., Kik, M., Rutteman, G.R., Teske, E., 2002. Prognostic significance of a new histologic grading system for canine osteosarcoma. Vet. Pathol. 39, 240-246.
Kow, It, Thamm, D.H., Terry, J., Grunerud, It, Bailey, S.M., Withrow, S.J., Lana, S.E., 2008. Impact of telomerase status on canine osteosarcoma patients. J. Vet. Intern. Med. 22,1366-1372.
Kurzman, I.D., MacEwen, E.G., Rosenthal, R.C., Fox, L.E., Keller, E.T., Helfand, S.C., Vail, D.M.,
Dubielzig, R.R., Madewell, B.R., Rodriguez Jr., CO., 1995. Adjuvant therapy for osteosarcoma in dogs: results of randomized clinica l trials using combined liposome-encapsulated muramyl tripeptide and cisplatin. Clin. Cancer Res. 1,1595-1601.
Lau, B., Cole, S.R., Gange, S.J., 2009. Competing risk regression models for epidemiologic data. Am. J. Epidemiol. 170, 244-256.
Marshall, A., Altman, D.G., Holder, R.L., Royston, P., 2009. Combining estimates of interest in prognostic modelling studies after multiple imputation: current practice and guidelines. BMC Med. Res. Methodol. 9,57.
McNeill, C.J., Overley, B., Shofer, F.S., Kent, M.S., Clifford, C.A., Samluk, M., Haney, S., Van Winkle, T.J., Sorenmo, K.U., 2007. Characterization of the biological behaviour of appendicular osteosarcoma in Rottweilers and a comparison with other breeds: a review of 258 dogs. Vet. Comp. Oncol. 5, 90-98.
Moore, A.S., Dernell, W.S., Ogilvie, G.It, Kristal, O., Elmslie, R., Kitchell, B., Susaneck, S., Rosenthal, R., Klein, M.It, Obradovich, J., Legendre, A., Haddad, T., Hahn, It, Powers, B.E., Warren, D., 2007. Doxorubicin and BAY 12-9566 for t
he treatment of osteosarcoma in dogs: a randomized, double-blind, placebo-controlled study. J. Vet. Intern. Med. 21, 783-790.
Morello, E., Vasconi, E., Martano, M., Peirone, B.,Buracco, P., 2003. Pasteurized tumoral autograft and adjuvant chemotherapy forthe treatment of canine distal radial osteosarcoma: 13 cases. Vet. Surg. 32,539-544.
Norrdin, R.W., Powers, B.E., Torgersen, J.L., Smith, R.E., Withrow, S.J., 1989. Characterization of osteosarcoma cells from two sibling large-breed dogs. Am. J. Vet. Res. 50,1971 -1975.
Phillips, B., Powers, B.E., Dernell, W.S., Straw, R.C., Khanna, C, Hogge, G.S., Vail, D.M., 2009. Use of single-agent carboplatin as adjuvant or neoadjuvant therapy in conjunction with amputation for appendicular osteosarcoma in dogs. J. Am. Anim. Hosp. Assoc. 45,33-38. R Development Core Team, 2013. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
Riley, R.D., Lambert, P.C., Abo-Zaid, G., 2010. Meta-analysis of individual participant data: rationale, conduct, and reporting. BMJ 340, c221.
Rowell, J.L., McCarthy, D.O., Alvarez, C.E., 2011. Dog models of naturally occurring cancer. Trends Mol. Med. 17,380-388.
Ru, G., Terracini, B., Glickman, L.T., 1998. Host related risk factors for canine osteosarcoma. Vet. J. 156,31-39.
Satagopan, J.M., Ben-Porat, L, Berwick, M., Robson, M., Kutler, D., Auer-bach, A.D., 2004. A note on competing risks in survival data analysis. Br. J. Cancer 91,1229-1235.
Sottnik, J.L., Rao, S., Lafferty, M.H., Thamm, D.H. , Morley, P.S., Withrow, S.J., Dow, S.W., 2010. Association of blood monocyte and lymphocyte count and disease-free interval in dogs with osteosarcoma. J. Vet. Intern. Med. 24,143
Spodnick, G.J., Berg, J., Rand, W.M., Schelling, S. H., Couto, G., Harvey, H.J., Henderson, R.A., MacEwen, G., Mauldin, N., McCaw, D.L., 1992. Prognosis for dogs with appendicular osteosarcoma treated by amputation alone: 162 cases
(1978-1988). J. Am. Vet. Med. Assoc. 200,995-999.
Sterne, J.A., White, I.R., Carlin, J.B., Spratt, M., Royston, P., Kenward, M.G., Wood, A.M., Carpenter, J.R., 2009. Multiple imputation for missing data in epidemiological and clinical research: potential and pitfalls. BMJ 338, b2393.
Stewart, LA, Parmar, M.It, 1993. Meta-analysis of the literature or of individual patient data: is there a difference? Lancet 341,418-422.
Steyerberg, E.W., Vickers, A.J., Cook, N.R., Gerds, T., Gonen, M., Obuchowski, N., Pencina, M.J., Kattan, M.W., 2010. Assessing the performance of prediction models: a framework for traditional and novel measures. Epidemiology 21,128-138.
Straw, R.C., Withrow, S.J., Richter, S.L., Powers, B.E., Klein, M.It, Postorino, N.C., LaRue, S.M., Ogilvie, G.It, Vail, D.M., Morrison, W.B., 1991. Amputation and cisplatin for treatment of canine osteosarcoma. J. Vet. Intern. Med. 5, 205-210.
Therneau, T., 2012. A Package for Survival Analysis in S.
Vail, D.M., Kurzman, I.D., Glawe, P.C., O'Brien, M.G., Chun, R., Garrett, L.D., Obradovich, J.E., Fred III, R.M., Khanna, C, Colbern, G.T., Working, P.It, 2002. STEALTH liposome-encapsulated cisplatin (SPI-77) versus carboplatin as adjuvant therapy for spontaneously arising osteosarcoma (OSA) in the dog: a randomized multicenter clinical trial. Cancer Chemother. Pharmacol. 50,131-136.
Withrow, S.J., Wilkins, R.M., 2010. Cross talk from pets to people: transla-tional osteosarcoma treatments. ILARJ. 51, 208-213.
Share this article / Teilen Sie diesen Artikel